Ribosomal DNA internal transcribed spacers to estimate the proportion of Pisolithus tinctorius and Eucalyptus RNAs in ectomycorrhiza

Evolution of ribosomal internal transcribed spacers in Deuterostomia.

Sequences of ribosomal internal transcribed spacers (ITSs) are of great importance to molecular phylogenetics and DNA barcoding, but remain unstudied in some large taxa of Deuterostomia. We have analyzed complete ITS1 and ITS2 sequences in 62 species from 16 Deuterostomia classes, with ITS sequences in 24 species from 11 classes initially obtained using unannotated contigs and raw read sequence...

Rapid detection, discrimination and quantification of Theileria orientalis types using ribosomal DNA internal transcribed spacers

Changes in protein biosynthesis during the differentiation of Pisolithus - Eucalyptus grandis ectomycorrhiza

Protein biosynthesis in Pisolithus Eucalyptus grandis ectomycorrhiza was related to the stage of ectomycorrhizal development using two-dimensional polyacrylamide gel electrophoresis of proteins labelled by in vivo incorporation of 35S radiolabelled amino acids. Nineteen-day-old seedlings were radiolabelled and the primary root was divided into I-cm segments. With increasing distance from the ti...

Low variation in ribosomal DNA and internal transcribed spacers of the symbiotic fungi of leaf-cutting ants (Attini: Formicidae).

Leaf-cutting ants of the genera Atta and Acromyrmex (tribe Attini) are symbiotic with basidiomycete fungi of the genus Leucoagaricus (tribe Leucocoprineae), which they cultivate on vegetable matter inside their nests. We determined the variation of the 28S, 18S, and 5.8S ribosomal DNA (rDNA) gene loci and the rapidly evolving internal transcribed spacers 1 and 2 (ITS1 and ITS2) of 15 sympatric ...

Detection of Leptosphaeria Korrae with Pcr and Primers from the Ribosomal Internal Transcribed Spacers

Leptosphaeria korrae, the causal agent of necrotic ring spot, is a destructive patch disease of Kentucky bluegrass. To develop a rapid molecular test for the detection of this pathogen, an assay based on the polymerase chain reaction was developed utilizing the internal transcribed spacer region 1 (ITS 1) of L. korrae ribosomal DNA. DNA sequence comparison showed 94.8% similarity of the ITS 1 r...